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Botany

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23 Jan 2009 17:40:28 IST

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does eukaryotic cells have restriction enzymes?

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does eukaryotic cells have restriction enzymes?

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N.P. Singh

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Joined: 17 Dec 2008

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24 Jan 2009 18:31:30 IST

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MOST restriction endonucleases are prokaryotic in origin. However, there are several found in eukaryotic cells, including our own. In eukaryotes they are not referred to as restriction enzymes, just endonucleases. An example of an endonuclease in eukaryotes is Apn1, isolated from yeast. This enzyme helps prevent DNA damage from environmental agents. In prokaryotes, restriction enzymes actually restrict the proliferation of viruses by cleaving their nucleic acids at specific base-pair sequences. . Eukaryotic endonucleases may not all help in restricting invading nucleic acids and in fact perform many distinct "jobs". That is probably why they are never referred to as restriction enzymes.
Restriction enzymes are DNA-cutting enzymes found in bacteria (and harvested from them for use). Because they cut within the molecule, they are often called restriction endonucleases.A restriction enzyme recognizes and cuts DNA only at a particular sequence of nucleotides. For example, the bacterium Hemophilus aegypticus produces an enzyme named HaeIII that cuts DNA The cut is made between the adjacent G and C the Nobel Prize in Medicine was awarded, in 1978, to Daniel Nathans, Werner Arber, and Hamilton Smith for the discovery of restriction endonucleases.Their discovery led to the development of recombinant DNA technology

vignesh the cooler version of newton

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25 Jan 2009 16:20:59 IST

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eukaryotes dont need restriction enzymes as we have RNA inactivation defending our cells......and i havent heard of any eukaryotic restriction endonuclease yet...

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