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Botany

Blazing goIITian

Joined:	3 Jun 2008
Post:	695

21 Jan 2009 11:11:36 IST

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wat is cDNA ???

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wat is cDNA ???

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JPS

Blazing goIITian

Joined: 21 Nov 2008

Posts: 1020

21 Jan 2009 12:35:44 IST

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cDNA means complementary DNA. they are prepared from the mRNA by using reverse transcriptase. this is used mainly to clone eukaryotic genes in prokaryotes.However, as eukaryotic DNA has introns, and since prokaryotes lack the machinery to splice them, the splicing of eukaryotic DNA must be done prior to adding the eukaryotic DNA into the host. So, usually a mature mRNA is taken obtained after splicing instead of the hnRNA.

>Devansh Jain< Hallelujah.......!!!!!!

Blazing goIITian

Joined: 24 Dec 2007

Posts: 467

21 Jan 2009 12:38:55 IST

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complementary DNA (cDNA) is DNA synthesized from a mature mRNA template in a reaction catalyzed by the enzyme reverse transcriptase.[1] cDNA is often used to clone eukaryotic genes in prokaryotes. cDNA is also produced by retroviruses (such as HIV-1, HIV-2, Simian Immunodeficiency Virus, etc.) which is integrated into its host to create a provirus.

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