Spray Reagents & their applications-PPPPPPLLLLLLZZZZ gve it a reading.   8 Nickels awarded! Tagged with:  academic    posted on 7 Jun 2007 10:27:12 IST
Ammonia 25% w/v in water.For neutralizing ninhydrin fixer for chromatograms and numerous other applications. Aniline-Diphenylamine 1.8% w/v aniline and 1.8% w/v diphenylamine in acidified acetone. For detecting reducing sugars. Antifoam 25% antifoam reagent in methylene chloride. For preventing/dispersing foam. Antimony Trichloride 22% w/v antimony trichloride in chloroform. For detecting vitamin A, steroid glycosides and related compounds. Bromocresol Green 0.04% w/v bromocresol green in ethanol. For detecting organic acids and bases. Chloramine-T 5% of reagent in 0.5% sodium hydroxide. For detection of phenolic compounds. Cupric Acetate 3% w/v cupric acetate and 15% w/v phosphoric acid. For detecting prostaglandins. a-Cyclodextrin 1% w/v of the reagent in ethanol. Distinguishes saturated from unsaturated lipids. 2 Dichlorofluorescein 0.2% w/v of the reagent in ethanol. For detecting saturated and unsaturated lipids. 4-Dimethylaminobenzaldehyde (Ehrich reagent) 1% w/v of the reagent in acidified metganol. For detecting amines, indoles, and ergot alkaloids. 4- Dimethylaminocinnamaldehyde 0.2% w/v of the reagent in acidified ethanol. For detecting indoles. N,N-Dimethyl-p-phenylenediamine dihydrochloride 1% w/v of the reagent in acidified methanol. For detecting organic peroxides. Diphenylcarbozone/Mercuric sulphate DPC is 0.01% in chloroform. Mercuric sulphate is 0.01% in dilute sulphuric acid. For detection of barbiturates. Diphenylhexatriene 0.03% of the reagent in chloroform. For detecting lipids. Dragendorffs reagent. 0.11% potassium iodide and 0.6millimole bismuth subnitrate in 3.5Macetic acid. For detecting alkaloids and quaternary nitrogen compounds. Ferric chloride-potassium ferricyanide 3% solutions in 2M hydrochloric acid For detecting aromatic amines and phenolic compounds. Ferric chloride 2.7% w/v of salt in 2M hydrochloric acid. For detecting phenols and phenolic acids. Fluorescamine 0.005% of the reagent in acetone. For detecting primary amino acids and amines. HPT Reagent 0.025% w/v 8-hydroxy-1,3,6-pyrenetrisulphonic acid trisodium salt in methanol. For detecting bile acids. Iodoplatinate 0.15% w/v potassium chloroplatinate and 3% potassium iodide in dilute hydrochloric acid. For detecting alkaloids, amines, and organic nitrogen compounds. Iodoplatinic acid 3ml. of 10% chloroplatinic acid diluted to 100ml.with water, then add 100ml. of 6% potassium iodide. For detection of alkaloids, amines, analgesics and biotin. Isatin 0.2% isatin in methanol. For detecting proline, hydroxyproline and other amino acids and peptides. Molybdenum blue 1.3% w/v molybdenun oxide in 4.2M sulphuric acid. For detecting phospholipids and related compounds. Ninhydrin 0.2% w/v ninhydrin in ethanol.or in 6% acetone. For detecting amino acids, amines, and amino sugars.(amphetamines) Ninhydrin Fixer reagent Acidified ethanol containing 1% saturated cupric nitrate. Fixative for ninhydrin chromatograms. N-4-Nitrobenzyl-N-n-propylamine hydrochloride (NBPA) 0.2 millimole reagent For detection of isocyanates. Orcinol Ferric Chloride (Bial's reagent) 0.9% w/v ferric chloride and 0.55% w/v orcinol in acidified ethanol. For detecting sugars,glycosides, and sulfolipids. Oxime (8-hydroxyquinoline) 0.25g in 8:20 etanol/water For detection of cations, AL, Cr, Fe, Co, Ni, Mn, Zn. Phosphormolybdic acid 5% of reagent in Propan 2-ol For detection of Fatty acid methyl esters, keto acids, lipids, steroids, and sulphated bile acids. Phosphoric acid / cupric acetate 3% cupric acetate w/v in 8% phosphoric acid v/v. For detection of Phospholipids. Rhodamine B 0.25% of regent in ethanol. For detecting lipids, and For detecting metals (Au, Bi, Cd, Fe, Hg, Mo, TI, V, and W.) Rhodamine 6G 0.01% w/v rhodamine 6G in methylene chloride. For detecting lipids. Rubeanic acid (sodium dithioxide) 0.1% in ethanol For detection of cations gb111A and 111B Sulphuric acid spray reagent 5% w/v of the acid in ethanol. Charring reagent for organic compounds. For detection of bile acids CHROMATOGRAPHY SPRAY INDEX Spray reagent selection guide Acids organic Bromocresol Green Aldehydes Fuchsin Alkaloids Dragendorff's Iodoplatinate Amines Iodoplatinate Ehrich's Ninhydrin Amino acids and related compounds Ninhydrin Antioxidants Phosphomolybdic acid BARBITURATES Mercuric diphenylcarbazone FLAVANOIDS Antimony trichloride GLYCOPIDS Bial's Orcinol Diphenylamine LIPIDS Antimony trichloride Bial's Orcinol Bromothymol blue Cupric acetate 2,7-Dichlorofluorescein Diphenylamine Dragendorff's Molybdenum blue Ninhydrin Potassium- Dichromate/H2SO4. Rhodamine B Phodamine 6G PHENOLS Phosphormolybdic acid Rhodamine B STEROIDS Antimony pentachloride Antimony trichloride Phosphormo;ybdic acid TERPENES Antimony pentachloride VITAMINS Antimony pentachloride Iodoplatinate CHARRING REAGENT Potassium dichromate/H2SO4 Hope u fnd it usfl frnds!!!!	About the Author: pirate1_from_jee (596) Scorching goIITian  108  [136 rates] total posts: 211     Offline
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comment by striker    (posted on 8 Jun 2007 10:21:58 IST) NICE WORK MISS
comment by dpgol88    (posted on 8 Jun 2007 10:25:53 IST) Itni chemistry to maine jee ke liye bhi nahi padhi thi lol! NEWAZ, GOOD WORK!

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