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It is very simple....
It is a method to find transforment from nontransforment DNA during rDNA technology.
During rDNA technology,desired gene is inserted on a recognation site of selective marker(tetracycline,amphicilin resistant gene etc...)of vector(say plasmid).Thus that gene(tetracycline,amphicilin resistant gene etc...) will be inactivated.This is called insertional inactivation.
So, transformant(recombinant DNA) will not be resistant to that particular chemical(tetracycline,amphicilin etc)
If we insert desired gene in amphicillin resistant gene,recombinants will survive in tetracycline but not in amphicillin.And the nontransformants will survive in both.Thus we can seperate transforment fro nontransforment.But this is a cumbersome process.
*If u understood rate me......
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Moderation Team
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